New research from scientists at 新澳门六合彩内幕信息 Davis Health provides clues for how friendly bacteria in the gut 鈥 probiotics 鈥 may help eradicate bacterial pathogens like Salmonella by competing with them for needed resources.
, published May 13 in Cell Host & Microbe, shows that the availability of needed nutrients alone doesn鈥檛 define where bacteria 鈥 including pathogens like Salmonella 鈥 can survive and thrive in the gut.
鈥淭hese insights provide a better understanding of the nutritional basis of intestinal colonization and can help inform efforts to develop probiotics to combat infection,鈥 said first author , a doctoral candidate in Professor Andreas 叠盲耻尘濒别谤's lab at the 新澳门六合彩内幕信息 Davis Department of Medical Microbiology and Immunology.
Humans are limited to using oxygen to generate energy. Microbes, though, have evolved energy mechanisms that can 鈥渂reathe鈥 different compounds and elements, such as nitrates. These mechanisms allow microbes to survive in many different environments.
In the study, the researchers looked at a friendly strain of bacteria, Escherichia coli strain Nissle 1917 (E. coli), and Salmonella, a harmful strain of bacteria that can cause gastrointestinal illness in humans and animals. (Some strains of E. coli , but most are harmless.)
Microbes use nitrate for energy
Nitrate is best known as a food preservative, but it is also an important resource for gut microbes. The nitrate from food sources is absorbed in the small intestine. Nitrate is also available in the gut as byproduct of everyday metabolic processes. It is typically at very low levels but increases during inflammation.
Both bacteria strains in the gut use nitrate for energy. But the researchers found that Salmonella can only use nitrate generated by phagocytes, a specific type of immune cells the body sends to repair wounded or infected tissues.
The E. coli uses nitrate generated by both gut cells 鈥 epithelial tissue 鈥 and phagocytes, meaning that it can compete with the Salmonella for its energy source.
Liou compared these different sources of nitrates to different 鈥渞estaurants鈥 where the microbes can obtain the resources they need to grow.
鈥淭he ability of E. coli Nissle to dine at 鈥榬estaurants鈥 generated by phagocytes and compete with the pathogen for resources was essential for the probiotic to confer protection against Salmonella,鈥 Liou said.
When the researchers infected mice with Salmonella, it triggered the expected inflammation in the gut, resulting in immune cells 鈥 phagocytes 鈥 being brought into the intestinal lumen, the layer that lines the intestines. Salmonella was able to find a niche in the lining of the intestines rich in phagocytes and exclusively used the nitrates generated by these immune cells.
Sensors limit where Salmonella can go
One puzzling aspect of the competition between the probiotic E. coli and the disease-inducing Salmonella was why the Salmonella did not utilize the nitrate produced by the healthy gut tissue. Why did it exclusively use nitrate produced by the immune-response tissue, limiting the number of places it could 鈥渄ine鈥?
The researchers found the answer in the sensors of Salmonella that allow the bacteria to sniff out its surroundings and move toward a more favorable environment, known as chemotaxis receptors. They steered the pathogen away from the epithelial-derived nitrate and toward the inflammatory phagocyte cells.
Essentially, the Salmonella did not have a sensing mechanism that would steer it toward the healthy epithelial tissue, even though that tissue also created nitrates. The probiotic E. coli lacks chemotaxis receptors, which allows it to occupy both niches and compete against the pathogen in the latter鈥檚 favored environment.
鈥淏y exploring the 鈥榬estaurants鈥 inside your colon, our research identifies properties of probiotics that protect us from infection,鈥 said , who is senior author of the study. 鈥淭hese insights provide a better understanding of the nutritional basis of intestinal colonization and can help inform efforts to develop probiotics to combat infection."
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(Cell Host & Microbe)
Lisa Howard is a senior public information officer at 新澳门六合彩内幕信息 Davis Health.