新澳门六合彩内幕信息

Unlocked Enzyme Structure Shows How Strigolactone Hormone Controls Plant Growth

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Malathy Palayam, Aleczander Young, Lior Tal, and Nitzan Shabek at the 新澳门六合彩内幕信息 Davis Department of Plant Biology are exploring the mechanism by which the plant hormone strigoractone regulates a massive network of genes controlling plant growth and architecture. (Shabek lab photo)

As sessile organisms, plants have to continually adapt their growth and architecture to the ever-changing environment. To do so, plants have evolved distinct molecular mechanisms to sense and respond to the environment and integrate the signals from outside with endogenous developmental programs.

New research from Nitzan Shabek鈥檚 laboratory at the 新澳门六合彩内幕信息 Davis College of Biological Sciences, published in Nature Plants, unravels the underlying mechanism of protein targeting and destruction in a specific plant hormone signaling pathway.

鈥淥ur lab aims at deciphering sensing mechanisms in plants and understanding how specific enzymes function can be regulated at the molecular levels鈥 said Shabek, assistant professor of biochemistry and structural biology in the Department of Plant Biology.  鈥淲e have been studying a new plant hormone signal, strigolactone, that governs numerous processes of growth and development including branching and root architecture.鈥

The work stems from a study by Shabek, published in Nature in 2018, unravelling molecular and structural changes in an enzyme, MAX2 (or D3) ubiquitin ligase. MAX2 was found in locked or unlocked forms that can recruit a strigolactone sensor, D14, and target for destruction a DNA transcriptional repressor complex, D53. Ubiquitins are small proteins, found in all eukaryotes, that 鈥渢ag鈥 other proteins for destruction within a cell. 

Photo art of padlock and key on a plant leaf
A permanently unlocked form of the MAX2 enzyme enabled the team to figure out how it works. (Nitzan Shabek)

To find the key to unlock MAX2 and to better understand its molecular dynamics in plants, postdoctoral fellows Lior Tal and Malathy Palayam, with junior specialist Aleczander Young, used an approach that integrated advanced structural biology, biochemistry, and plant genetics.

鈥淲e leveraged structure-guided approaches to systemically mutate MAX2 enzyme in Arabidopsis and created a MAX2 stuck in an unlocked form鈥, said Shabek, 鈥渟ome of these mutations were made by guiding CRISPR/Cas9 genome editing thus providing us a discovery platform to study and analyze the different signaling outputs and illuminate the role of MAX2 dynamics.鈥

Regulating a massive gene network

They found that in the unlocked conformation, MAX2 can target the repressor proteins and biochemically decorate them with small ubiquitin proteins, tagging them for destruction. Removing these repressors allows other genes to be expressed 鈥 activating a massive gene network that governs shoot branching, root architecture, leaf senescence, and symbiosis with fungi, Shabek said.  

Sending these repressors to the proteasome disposal complexes requires the enzyme to relock again. The team also showed that MAX2 not only target the repressors proteins, but once it is locked the strigolactone sensor itself gets destroyed, returning the system to its original state.

Finally, the study uncovered the key to the lock, an organic acid metabolite that can directly trigger the conformational switch.

鈥淏eyond the implication in plants signaling, this is the first work that placed a primary metabolite as a direct new regulator of this type of ubiquitin ligase enzymes and will open new avenues of study in this direction,鈥 Shabek said.

Additional coauthors on the paper are specialist Mily Ron and Professor Anne Britt, Department of Plant Biology. The study was supported by NSF CAREER and EAGER grants to Shabek. X-ray crystallography data was obtained at the Advanced Light Source, Lawrence Berkeley National Laboratory, a U.S. Department of Energy user facility.  

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 (Nature Plants)

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