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New DNA sequencing technology has been used to identify the virus that causes red blotch, a disease that discolors grape leaves in fall and lowers sugar levels in grapes, agricultural nursery managers were told today during an annual industry update at the University of California, Davis.

The virus is of particular concern to wine grape growers, whose grapes must reach a certain sugar content level before they are suitable for winemaking.

“The most urgent research need now is to determine how the virus spreads,” said Deborah Golino, a Cooperative Extension plant disease specialist and director of the �°������ϲ���Ļ��Ϣ Davis-based Foundation Plant Services.

“Due to the distribution of the virus in many parts of the United States and evidence that it can be transmitted by grafting, we suspect that red blotch disease is widespread wherever grapes are grown,” Golino said.

Golino encouraged vineyard owners and managers to evaluate their vineyards for red blotch disease as they would for any other viruses. Symptoms include blotches of pink or red veins on green leaves in the fall, when grape leaves would normally be turning a uniform gold color. Growers also might notice that their grapes are slow to develop sugar levels sufficient for winemaking, with some grapes never fully maturing.

“If there are visual signs of red blotch, as well as poor sugar development, growers should test their vines for both red blotch and leafroll virus because the symptoms of the two viruses are so similar,” Golino said.

She reported that red blotch disease has been identified among both young and mature grape vineyards in California, New York, Virginia, Maryland, Pennsylvania, Texas and Washington. A virus almost genetically identical to red blotch also was found in Canada.

While the virus, also called grapevine red blotch-associated virus or GRBaV, likely can be found in all types of grapes, including rootstock, and table and raisin grapes, it was first detected in wine grapes because they are carefully monitored for sugar content to determine harvest date.

Presence of the virus in a vine can be confirmed using an extremely sensitive laboratory test known as polymerase chain reaction, or PCR, to detect small amounts of genetic material. This analytical process uses an amplification technique that multiplies the existing DNA and similar genetic material to identify the virus.

“If the vineyard tests positive for red blotch, the grower needs to decide, from an economic standpoint, when is the best time to remove the diseased vines and replant the vineyard,” Golino said. “This is always a complex decision, and there is no one-case-fits-all answer.”

Golino said that the Foundation Plant Services’ Classic Foundation vineyard block, used for producing disease-free scion and rootstock vines, has been partially tested and appears to have a very low-level incidence of red blotch virus. Only three of the 1,600 vines tested to date were found to have the virus. Testing of all of the vines in the Classic Foundation vineyard, the source of the majority of grapevine nursery stock in California, is slated for completion in the months to come.

“The good news is that our new Russell Ranch Foundation Vineyard block has been tested, and there is no sign of red blotch virus in any of those vines,” Golino said. This vineyard is propagated exclusively with vines that have been through tissue-culture therapy to exclude red blotch and other viruses. Test records from the Foundation Plant Services vineyards are available .

Golino cautioned that many more new viruses and other microbes are likely to be found in grapevines in the next few years, thanks to powerful new DNA sequencing technology.

“Some of those will be disease agents, some beneficial, and some neutral,” she said. “We will have our work cut out for us in understanding the role of these microbes, but the ultimate result will be increased ability to create superior vineyards.”

“The appearance of red blotch virus underscores the vital importance of the Foundation Plant Services and adequate funding for USDA’s National Clean Plant Network,” said John Aguirre, president of California Association of Winegrape Growers. “Growers need clean plant material to meet winery demands for increased winegrape production and improved quality.” 

Golino said that progress on identifying and characterizing red blotch has been a collaborative effort between many university-based researchers over the years.

Red blotch disease was first recognized in 2008 in a Napa Valley vineyard by Jim Wolpert, a �°������ϲ���Ļ��Ϣ Davis-based Cooperative Extension viticulturist, and Mike Anderson, a viticulture researcher and manager of �°������ϲ���Ļ��Ϣ Davis’ Oakville Experiment Station.

Two �°������ϲ���Ļ��Ϣ Davis scientists — Mysore Sudarshana , a U.S. Department of Agriculture researcher in the plant pathology department, and Maher Al Rwahnih, a researcher at Foundation Plant Services — teamed up in an effort to identify the virus causing red blotch disease. Al Rwahnih was an early user of next-generation, or high-throughput DNA sequencing technology.

During today’s meeting, Golino announced that California rootstock nurserymen acted last week to assist the California viticulture industry with research addressing GRBaV. The California Grape Rootstock Research Foundation, which funds research to enhance California viticulture and the grape nursery industry, has agreed to provide seed money to jumpstart research on the virus.

More information on red blotch disease is available on the .

Foundation Plant Services is a self-supporting unit of the College of Agricultural and Environmental Sciences at �°������ϲ���Ļ��Ϣ Davis. The service, dedicated to the distribution of disease-tested, true-to-identity plant materials produced by �°������ϲ���Ļ��Ϣ researchers, plays a key national and international role in distributing new crop varieties and healthy planting stocks.